We are interested in how loss of TSC affects neuronal function by studying the molecular pathways that are disrupted in neurons. We are using a range of model systems such as animal models, neuronal models in vitro, and iPSC-derived cortical neurons to study these pathways. In addition, we are using several different techniques such as single cell sequencing. We have found that there are several candidate pathways that are disrupted including several transcription factors and RNA binding proteins.